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ARS Home » Research » Publications at this Location » Publication #86026
Title: DEVELOPMENT OF A BLOCKING ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTION OF SERUM ANTIBODIES TO O157 ANTIGEN OF ESCHERICHIA COLI

Author
item Laegreid, William
item Keen, James
item Kwang, Hwei Sing
item Hoffman, Mark
item Bosworth, Brad

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 10/7/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The O157 antigen of Escherichia coli shares structural elements with lipopolysaccharide (LPS) antigens of other bacterial species, notably Brucella abortus and Yersinia enterocolitica O9. These similarities confound interpretation of assays for anti-O157 antibodies. To address this problem, a monoclonal antibody specific for O157, designated 13B3, was derived which did not cross-react with Brucella or Yersinia spp. A cELISA was designed using highly purified E. coli O157:H7 LPS as antigen and 13B3 as the competing antibody. The cELISA had greater sensitivity and specificity than the indirect ELISA (iELISA) detecting anti-O157 antibodies in sera from cattle experimentally inoculated with O157:H7. Sera from naive heifers had no detectable anti-O157 titers by cELISA before or after Brucella abortus Strain 19 vaccination, while 30% of pre-vaccination and 75% of post-vaccination sera were positive by iELISA. The cELISA is a sensitive and specific method for the detection of serum antibodies caused by exposure to E. coli O157.