Technical Abstract: The soybean cyst nematode (SCN), Heterodera glycines Ichinohe is a major pest of the soybean worldwide and is responsible for millions of dollars worth of damage in the USA. There are numerous races of SCN and control of damage can be difficult. We studied the differential expression of genes that are induced upon inoculation of resistant and susceptible soybean cultivars with soybean cyst nematode, race 3. Thirty combinations ofprimers were used to generate differential display bands from the different plant tissue samples. We cloned and sequenced 35 bands that are present in the resistant inoculated plant but not in the susceptible, uninoculated or wounded controls. According to the timing of gene expression, these cDNAs can be assigned to early (6-24 hours), intermediate (24-48 hours) or late (48-144 hours) stages of the nematode infection process. Genes that are induced include those involved in regulation of gene expression such as transcription factors and different nucleotide binding proteins, and general defense response genes such as chitinases and peroxidases is detected. The study of differential gene expression throughout the nematode infection process is of importance to plant scientists who are designing ways to make soybeans more resistant to SCN.