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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Publications at this Location » Publication #85199
Title: ANALYSIS OF ALIEN GENOME INTROGRESSION IN RICE USING GENOME-SPECIFIC REPETITIVE DNA PROBES

Author
item ASWIDINNOOR, H - BOGOR AGRICULTRUAL UNIV
item NELSON, R - IRRI, THE PHILIPPINES
item Gustafson, J

Submitted to: Asia Pacific Journal of Molecular and Biological Biotechniques
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/31/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary: Wild species of rice contain genes useful for increasing the productivity of cultivated rice. A serious problem for breeders interested in using the genetic potential of wild species is breeding the desired qualities into commercial varieties while eliminating the many undesireable traits. This study was designed to detect deleterious genes using DNA markers for "marker-assisted selection". Use of these molecular tools will favorably impact the crop improvement process.

Technical Abstract: Analysis of genome introgression from Oryza minuta, a wild rice species containing blast (Pyricularia grisea) resistance, into cultivated rice (O. sativa) was done by utilizing backcross progeny from a cross involving the two species. Southern blot hybridization established that three repetitive DNA probes isolated from O. minuta (pOm4, and pOmPB10) and O. alta (pOa237) were able to detect genome introgression from O. minuta in the backcross progeny. The results showed that the presence of the three sequences together in the progeny suggested either the close proximity of the sequences in the O. minuta genome, or that a fairly large amount of O. minuta genome had been introgressed into the backcross progeny, which was detected by the three probes. This suggested that more than one recombination event had taken place between the two species. However, neither of the three DNA probes showed any association with the presence or absence of blast resistance. The molecular markers were able to differentiate between resistant plants containing considerable amounts of unwanted O. minuta DNA and those still resistant, but containing undetectable amounts of O. minuta DNA. They were only useful as tools in helping to eliminate any linkage drag associated with the wild species O. minuta.