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United States Department of Agriculture

Agricultural Research Service

Title: Registration of Rel-1 and Rel-2 Sugarbeet Germplasms for Tissue Culture Genetic Manipulatioins

Author
item Saunders, Joseph

Submitted to: Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: November 30, 1997
Publication Date: N/A

Interpretive Summary: Beet sugar could be produced more economically if beneficial genes from other crops could be added more efficiently, or if novel beneficial traits could be efficiently selected at the cell level. REL-1 and REL-2 germplasms are better able than most sugarbeets to allow genetic changes produced at the cell level to be brought to the whole plant level and efficiently bred into modern hybrids because additionally they have genes for faster flowering. This accelerated development of new varieties is important to sugarbeet farmers and processors, as well as as seed suppliers, because of the reduced costs to produce sugar in each part of the chain in the sugarbeet industry.

Technical Abstract: Two F1 individuals with superior tissue culture characteristics for use in biotechnological genetic improvement have been produced and cloned for distribution to the seed industry. Both are diploid, annual for rapid floral induction, and self-fertile for easier seed production. REL-1 produces callus and subsequent shoots from nearly a hundred percent of the leaf discs plated on Murashige-Skoog medium with 1.0 mg/L 6-benzyladenine. Its fine textured suspension cultures are amenable for plating for cell selection. REL-1 is resistant to shoot vitreousness. REL-2 was produced as a hybrid of REL-1 and an intensely shoot regenerating individual from line EL45/2 (as female). REL-2 regenerates shoots more intensely than REL-1 and produces prolific numbers of somatic embryos. REL-1 was released to the public in 1987 and has already been used in some labs to produce beneficial mutants by cell selection, as well as transgenic plants. REL-2 has the potential to be more efficient in transgenic applications.

Last Modified: 11/28/2014
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