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Title: CONSERVATION AND IMMUNOREACTIVITY OF THE PERIPLASMIC 31 KILODALTON IRON- REGULATED PROTEIN (PIRP) IN PASTEURELLA HAEMOLYTICA AND PASTEURELLA TREHALOSI SEROTYPES.

Author
item Tabatabai, Louisa
item Frank, Glynn

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 11/10/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: We report that a major 31 kDa periplasmic iron-regulated protein expressed by all 12 serotypes of P. haemolytica, grown under conditions of iron- limitation, is expressed as alternate forms with molecular weights of 31 kDa and 28 kDa by the four serotypes of P. trehalosi. N-terminal sequencing of P. haemolytica and P. trehalosi periplasmic iron-regulated 31 kDa proteins show that the 31 kDa protein contains the N-terminal sequence EPKFV, whereas the 28 kDa protein contains the N-terminal sequence AQPFV. Immunoblots with rabbit antiserum prepared to the purified 31 kDa iron- regulated protein from P. haemolytica serotype A1 demonstrated the absence of the 31 kDa protein from P. trehalosi serotypes T3 and T4 and a faint reaction with P. trehalosi serotypes T10 and T15. Furthermore, the anti-31 kDa antibody showed a faint reaction with a protein of 28 kDa in P. trehalosi serotypes T3, T4, T10, and T15. These results suggest that the 28 8kDa and the 31 kDa proteins of P. trehalosi are related to the 31 kDa protein of P. haemolytica. The 28 kDa protein is absent from the P. haemolytica serotypes tested. It is not known whether the expression of the alternate forms of the 31 kDa periplasmic iron-regulated protein of P. haemolytica is related to the different diseases caused by these two species.