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United States Department of Agriculture

Agricultural Research Service

Title: Segregation of Microsatellite Loci in Haploid and Diploid Gametes of Musa

Authors
item Crough, Hutokshi - IITA HIGH RAINFALL SUBSTA
item Crouch, Jonathan - IITA HIGH RAINFALL SUBSTA
item Jarret, Robert
item Cregan, Perry
item Ortiz, Rodomiro - IITA HIGH RAINFALL SUBSTA

Submitted to: Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 25, 1998
Publication Date: N/A

Interpretive Summary: Traditional plantain improvement strategies are generally based on the breeding of wild plantain-related species which are subsequently intermated with cultivated plantain. In this study, molecular markers were utilized to demonstrate that plantain improvement can also be accomplished by intermating various cultivated plantain types directly.

Technical Abstract: This study was conducted to analyze the genetic constitution of tetraploid hybrids in order to characterize the nature of the gametes from which they were derived. Parental genotypes were screened with 33 primer pairs specific to different Musa microsatellite loci. Primers which detected polymorphisms between the parental genotypes were used to screen a population of 14 full-sib tetraploid hybrids. A subset of primers was als used to screen a population generated by self-pollination of the parental diploid banana. During analysis of the tetraploid hybrid population, 75% of the alleles donated by the maternal genotype (Obino 1'Ewai) were observed to segregate. These data demonstrate the occurrence of recombination during the formation of 2n megaspores in triploid plantain. Analysis of the paternal diploid banana accession (Calcutta 4) and its selfed progeny suggests that, in contrast to previous reports, this accession is highly heterozygous. These data demonstrate the importance of genetic characterization of Musa breeding schemes.

Last Modified: 8/2/2014
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