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ARS Home » Pacific West Area » Aberdeen, Idaho » Small Grains and Potato Germplasm Research » Research » Publications at this Location » Publication #82025
Title: RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) ANALYSIS IN BARLEY PLANTS DERIVED FROM ANTHER CULTURE

Author
item Hang, An
item Burton, Charlotte
item SATTERFIELD, KATHY - UNIVERSITY OF IDAHO

Submitted to: Cytologia
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/3/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary: The cells that will give rise to pollen grains in an anther of a plant are microspores. When anthers are cultured on an artificial medium, the microspores can develop into plants, but plants produced in this manner contain only one set of chromosomes and are called haploid plants. During culture, the haploid chromosome set can be spontaneously doubled, resulting gin doubled-haploid plants. All doubled-haploid plants derived from the anthers of a plant theoretically should be exactly the same because they came from one set of chromosomes carrying the same genes. We used polymerase chain reaction (PCR) technique to detect variation at the DNA level in the chromosomes of 123 doubled-haploid plants produced from anther culture of six barley varieties. We found that anthers cultured on artificial medium can show DNA structure changes creating variation in regenerated plants. The degree of variation depended on the barley variety. This variation can be used for selection of desirable traits for crop improvement.

Technical Abstract: Random amplified polymorphic DNA (RAPD) was used to study DNA variation in 123 green, doubled-haploid plants derived from anther culture of six barley cultivars, including 'Andre', 'Harrington', 'Hector', 'Moravian III', 'Morex', and 'Otis'. Polymorphic fragments were found among derived lines and between cultivar and derived lines with 16 of 22 primers studied. The frequencies of polymorphism varied between derived lines. Lines derived from Moravian III showed polymorphism with 13 primers, while Hector and Hector derived lines were monomorphic with 22 primers studied. This indicated that DNA may be modified during anther culture and the degree of variation depended on the genotype of each cultivar. This variation may be used for selection of desirable characteristics in a breeding program.