|Evans Marks, Loreene|
|Rosato, S - STATE OF OR, CORVALLIS,OR|
|Mueller Warrant, George|
|Hines, W - NC STATE UNIVERSITY|
Submitted to: Grass Breeders Work Planning Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: March 17, 1997
Publication Date: N/A
Interpretive Summary: Basically, there are two types of molecular marker DNA analyses; 1) those that use restriction cleavage and marked probes (e.g., RFLP-- restriction fragment length polymorphism) and 2) those based on polymerase chair reactions (PCR) that multiple copies of DNA segments (e.g., RAPD--random amplified polymorphic DNA). The two analysis examples are very different, but many techniques have been developed recently that are modifications of each, and some, such as AFLP (amplified fragment length polymorphism), combine elements of both. RAPD analysis in cross breeding populations adds a difficult dimension to interpretation that is not found in many other crops where DNA analyses have been utilized. Our laboratory work is focused on maintaining genetic integrity of grass cultivars during seed multiplication. Using molecular DNA markers to detect genetic shifts and contamination is becoming a valuable tool for us. In this report, we give brief examples of three of our studies. These studies are still in progress so conclusions were not provided here. These examples were presented to define areas where we are having difficulties and may be useful to others if they plan to embark on similar research.
Technical Abstract: This is a short technical article describing a new method of molecular techniques and analytical procedures. It is not abstracted because of the length of the manuscript and it is only intended for a scientific article for grass breeders and geneticists.