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United States Department of Agriculture

Agricultural Research Service

Title: An Evaluation of Tissues Used As Target Cells in Genetic Transformation of Cereals.

Authors
item Bommineni, Venkata
item JAUHAR, PREM

Submitted to: Maydica
Publication Type: Review Article
Publication Acceptance Date: April 9, 1997
Publication Date: N/A

Interpretive Summary: Tissues amenable to in vitro manipulations as well as rapid regeneration of plants are some of the essential factors in plant transformation. Only a few years ago, cereals were considered recalcitrant for transformation. However, genetic transformation of cereals has become a routine procedure and there are numerous reports of transgenic cereals throughout the world. Among different explants, three types of tissues are used routinely as target cells in genetic transformation of cereals. Embryogenic calli and cell suspensions are more frequently used in biolistic transformation, whereas protoplasts from cell suspensions are commonly used in PEG-or electroporation-mediated transformation of cereals. Other organized tissues were also used to achieve stable transformation, but generally without much success. The utilization of several target materials is summarized, and their applications in cereal transformation are discussed.

Technical Abstract: Tissues amenable to in vitro manipulations as well as rapid regeneration of plants are some of the essential factors in plant transformation. Only a few years ago, cereals were considered recalcitrant for transformation. However, genetic transformation of cereals has become a routine procedure and there are numerous reports of transgenic cereals throughout the world. Among different explants, three types of tissues are used routinely as target cells in genetic transformation of cereals. Embryogenic calli and cell suspensions are more frequently used in biolistic transformation, whereas protoplasts from cell suspensions are commonly used in PEG-or electroporation-mediated transformation of cereals. Other organized tissues were also used to achieve stable transformation, but generally without much success. The utilization of several target materials is summarized, and their applications in cereal transformation are discussed.

Last Modified: 8/19/2014
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