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United States Department of Agriculture

Agricultural Research Service

Title: Cytoplasmic Male Sterility and Fertility Restoration in a Helianthus Annuuslandrace Pi432513

Authors
item Jan, Chao-Chien
item Vick, Brady

Submitted to: Proceedings Sunflower Research Workshop
Publication Type: Abstract Only
Publication Acceptance Date: February 15, 1997
Publication Date: N/A

Technical Abstract: A single male-sterile cytoplasm of H. petiolaris has been used world-wide for hybrid sunflower production over the past 20 years. This paper reports our recent discovery of a new cms source from Native American landrace PI432513, and the identification of its restoration genes. One male-sterile (ms) plant was identified in PI432513 in 1995 at Fargo, ND. This plant was spollinated with pollen from HA89, sib-pollinated by male-fertile (mf) plan of PI432513. All the five F1 plants from ms-PI432513 x HA89 were male sterile, and the ms-PI432513 x mf-PI432513 cross produced three mf and seven ms plants, indicating cytoplasmic male sterility and the existence of fertility restoration genes in mf-PI432513. Those cms plants were then pollinated by 13 restoration testers representing diverse genetic background, and progenies grown in the field in 1996. A low frequency of fertility restoration genes was recovered from Armavir, VNIIMK, P21, and a uniform fertility restoration by RHA265, RHA266, RHA274, RHA294, RHA296, and RHA801 was observed. This is the first cms H. annuus cytoplasm having a fertility restoration reaction similar to the classical H. petiolaris cytoplasm. It is very likely that the same gene or genes restoring classical cms are also restoring this new cms from H. annuus. The three male-fertile F1 plants of ms-PI432513 x mf-PI432513 were both self- pollinated, and sib-pollinated with cms PI432513 plants. The segregation ratio of 3 mf to 1 ms in F2 and 1 mf to 1 ms in BC1F1 strongly suggest a single dominant gene for fertility restoration.

Last Modified: 10/22/2014
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