ARS | Publication request: GENOMIC SUBTYPING OF EDWARDSIELLA ICTALURI ISOLATES FROM DISEASED CHANNEL CATFISH BY ARBITRARILY PRIMED POLYMERASE CHAIN REACTION.

Submitted to: American Society for Microbiology
Publication Type: Abstract Only
Publication Acceptance Date: September 9, 1997
Publication Date: N/A

Technical Abstract: Arbitrarily Primed Polymerase Chain Reaction (AP-PCR) utilizing a Enterobacterial Repetitive Intergenic Consensus PCR primer (ERIC II) to genomically subtype 19 isolates of Edwardsiella ictaluri from channel catfish from throughout the Southeastern United States, an American Type Culture Collection reference strain, and an isolate from a walking catfish, Clarias batrachus from Thailand. Four major subgroupings of E. ictaluri were observed. These subgroups were distributed with the following prevelences: subgroup 1 (55%), subgroup 4 (20%), 2 (15%), and 3 (10%). Biochemical analysis showed biochemical homogeneity among isolates and was not useful for discriminating between isolates.