Skip to main content
ARS Home » Midwest Area » West Lafayette, Indiana » Crop Production and Pest Control Research » Research » Publications at this Location » Publication #77485
Title: MOLECULAR AND CYTOGENETIC ANALYSIS OF BARLEY YELLOW DWARF VIRUS RESISTANT TRANSLOCATION LINES CONTAINING THINOPYRUM INTERMEDIUM CHROMOSOMAL SEGMENTS

Author
item Anderson, Joseph
item CRASTA, O - PURDUE UNIVERSITY
item FRANCKI, M - PURDUE UNIVERSITY
item BUCHOLTZ, D - PURDUE UNIVERSITY
item SHARMA, H - PURDUE UNIVERSITY
item OHM, H - PURDUE UNIVERSITY

Submitted to: Plant Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 1/5/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Wheat germplasm lacks true resistance to barley yellow dwarf virus (BYDV), the most significant viral pathogen of cereals worldwide. Soft red winter wheat containing the Thinopyrum intermedium 7E chromosome have been developed and determined to be resistant to BYDV. Translocation lines (TLs) have been developed by subjecting the monosomic alien substitution line to gamma-irradiation and monitoring the selfed progenies for chromosome number, segregation pattern of BYDV resistance and presence of Thinopyrum chromatin. The objectives of this research are qualitative and quantitative estimation of the presence of Thinopyrum chromatin in TLs, identification of the chromosomal segment containing BYDV resistance and identification of suitable translocation lines as germplasm for variety development and for use in targeted mapping strategies to isolate DNA markers associated with the BYDV resistance locus. We have utilized a comparative mapping strategy to characterize the TLs. DNA markers shown t be collinear across several species of Gramineae or mapped on wheat group 7 chromosomes were utilized in identifying 7E and 7D chromosomal segments in TLs. The presence or absence of the chromosomal segments containing these markers provided a precise characterization of the amount of 7E chromosomal segments translocated. The association of these markers with BYDV resistance in various TLs suggested that the BYDV(R) locus is present in the long arm of chromosome 7E. This molecular characterization has formed a sound basis for further use of suitable translocation lines in targeted mapping of BYDV resistance and in developing elite wheat cultivars resistant to BYDV.