|Ananiev, Evgueni - UNIVERSITY OF MINNESOTA|
|Riera-Lizarazu, Oscar - UNIVERSITY OF MINNESOTA|
|Phillips, Ronald - UNIVERSITY OF MINNESOTA|
Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: December 1, 1996
Publication Date: N/A
Technical Abstract: Maize (Zea mays L.) chromosome addition lines of oat (Avena sativa L.) recovered from oat x maize crosses serve as a means to clone individual maize chromosomes. These lines also provide a unique opportunity to produce by irradiation a series of oat-maize introgression lines with small segments of an individual maize chromosome added to an oat genome. Substantial differences between the oat and maize genomic DNA have enabled development of a highly effective screening procedure for detecting introgressed maize DNA using maize-specific dispersed repetitive DNA sequences. A cosmid library was constructed from DNA of a maize chromosome 9 addition line and screened with the help of a multiprobe composed of a mixture of maize repetitive sequences. A set of 250 maize-specific cosmid clones from maize chromosome 9 was used to determine the distribution of different repeated sequences and to isolate additional repeated sequences as well as a set of unique sequences specific for chromosome 9.