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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #76882
Title: MOLECULAR CHARACTERIZATION OF TRANSGENE INTEGRATION IN OAT

Author
item PAWLOWSKI, WOJCIECH - UNIVERSITY OF MINNESOTA
item TORBERT, KIM - UNIVERSITY OF MINNESOTA
item Rines, Howard
item SOMERS, DAVID - UNIVERSITY OF MINNESOTA

Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 12/1/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Integration of plasmid DNA carrying two marker genes was investigated in transgenic oat (Avena sativa L.) tissue cultures and plants that had been transformed by microprojectile bombardment. Ninety percent of the transformation events had at least one intact copy of one or both of the transgenes. Only in 3% of the tissue cultures and plants was one of the transgenes entirely missing. The intact transgene copies were accompanied by multiple truncated and rearranged fragments of transgenic DNA resulting in complex transgene integration patterns as indicated by the presence of several different size restriction fragments on Southern blot analysis. Complex transgene integration patterns suggested that transgene delivery to the plant cells and/or genomic integration was associated with extensive rearrangement and fragmentation of introduced DNA. All introduced transgene fragments were integrated into one genetic locus in the plant genome as indicated by transgene segregation patterns in the progeny. A possible mechanism of transgene integration resulting in such an arrangement of transgenic DNA will be proposed.