REGULATORY FUNCTION OF THE MAREK'S DISEASE VIRUS ICP27 GENE PRODUCT

Authors: REN, DELIN - MICHIGAN STATE UNIVERSITY; Lee, Lucy; COUSSENS, PAUL - MICHIGAN STATE UNIVERSITY

Submitted to: International Marek's Disease Symposium Abstracts and Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 9/7/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: MDV ICP27, an MDV immediate-early protein, is phosphorylated and predominantly localizes to nuclei of CEF cells infected with serotype-1 MDV or with a fowlpox virus recombinant expressing MDV ICP27. Transient expression assay indicates that MDV ICP27 possesses both intrinsic transactivation and transrepression activities. MDV ICP27 significantly transactivates MDV pp14 and pp38 bi-directional promoters, but strongly represses the MDV thymidine kinase (TK) early gene promoter. In addition, a heterologous RSV-LTR U3 promoter is also stimulated by MDV ICP27. In contrast to HSV-1 ICP27, MDV ICP27 shows now cooperative activity with MDV ICP4.