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Submitted to: Oily Press
Publication Type: Book / Chapter Publication Acceptance Date: 6/1/1997 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: The lipoxygenase (LOX) or oxylipin pathway is receiving increasing attention possibly because of its role in the physiological processes of plants, particularly as a defense against pathogens. At this time it seemed useful to review the methods available to analyze the various metabolites of the pathway. First, the pathway itself is reviewed briefly. Because most of the current interest has focused on the steps subsequent to LOX action, there is a section on substrate preparation, such as fatty acid hydroperoxides and other metabolites. Methods of recovery and separation are the next logical topic to review. This is followed by the preparation of useful derivatives, and often a series of derivatives can be a powerful tool if used in conjunction with spectral methods. In regard to spectroscopy, gas chromatography-mass spectroscopy (GC-MS) and nuclear magnetic resonance (NMR) are the most useful techniques, but other spectral methods can fill some gaps in structural analyses. The goal of preparing this chapter is to provide an easy-to-use guide for investigators who are not normally lipid chemists. |
