Technical Abstract: The collection of primitive accessions of cotton, Gossypium hirsutum L., contain genetic diversity. However, because many of the accessions are photoperiodic, this diversity is not readily useable by plant breeders. Sixteen accessions were crossed to Deltapine 16 and progeny with day-neutral flowering habit were selected in the F2 generation. These progeny were then backcrossed four times to their respective primitive accession parent and selected for day neutrality in the F2 following each backcross and advanced to the F5. The resulting day-neutral progenies (F5, BC1F5, BC2F5, BC3F5, and BC4F5) for the 16 accessions were evaluated for agronomic and fiber traits for three years. The objective of the present study was to determine genetic variances and correlations for the traits studied. Variation of accession generation was highly significant for all traits studied. Main effect of accessions or generations was significant. However, the magnitude was relatively small compared to the interaction variation. Lint yield had significant but small positive phenotypic correlations with lint percentage, boll size, and 2.5% span length, but not with the other traits studied. Highly significant positive correlations were found for effects of accession, generation, and accession generation between lint yield and lint percentage.