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United States Department of Agriculture

Agricultural Research Service

Title: Cloning of Cellulase Genes from Acidothermus Cellulolyticus

item Lastick, Stanley - DECEASED
item Tucker, Melvin -
item Grohmann, Karel

Submitted to: Patent Application
Publication Type: Other
Publication Acceptance Date: May 7, 1996
Publication Date: N/A

Interpretive Summary: A thermophilic bacterium Acidothermus cellulolyticus produces a cellulase enzyme system, which is stable almost to the boiling point of water and active in acidic media. The bacterium, however, secretes only relatively low levels of cellulolytic activity. Two DNA fragments containing a A. cellulolyticus cellulase genes were isolated and cloned on DNA vectors in a rapidly growing bacterium Escherichia coli. Successful expression of these thermophilic genes has been achieved in E. coli and over producing plasmid system has also been constructed.

Technical Abstract: A process is described for moving fragments that code for cellulase activity from the genome of A. cellulolyticus to several plasmid vectors and the subsequent expression of active cellulase activity in E. coli.

Last Modified: 4/22/2015
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