|Peterson, Terrance - PLNT SCI, NDSU, FARGO, ND|
Submitted to: International Crop Science Congress Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: July 30, 1996
Publication Date: N/A
Technical Abstract: Genetic engineering with genes of direct interest has been shown to accelerate plant improvement. Durum wheat (Triticum turgidum L., 2n = 4x = 28) is grown in the Northern Great Plains region of the US and is extensively used for making semolina and pasta. To improve durum germplasm by transgenesis, we established a Biolistic transformation method with isolated scutella as target cells and utilized bar (herbicide resistance gene) as a selectable marker in the gene construct pBARGUS (Bommineni et al., Plant Genome IV: 94, 1996). Plants recovered on selection medium with 5 mg**-L L-phosphinothricin (L-PPT) were monitored for gus expression and confirmed by Southern analysis in the next generation (T1). The transgenic plants in T0 were first selected by spot brush application of glufosinate herbicide at 120 mg**-L L-PPt, and T1 and T2 generations were raised from a randomly selected sample of seeds and sprayed with glufosinate to recover plants segregating for bar. Most of these plants proved positive for phosphinothricin acetyltransferase (PAT) activity. However, most plants selected for herbicide resistance showed inheritance of bar without expressing gus. These results were confirmed by PCR and Southern blot analysis.