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United States Department of Agriculture

Agricultural Research Service

Title: A Codominant Random Amplified Polymorphic DNA (Rapd) Marker Useful for Indirect Selection of Bgmv Resistance in Common Bean

Authors
item Urrea, Carlow - UNIV PUERTO RICO STUDENT
item Miklas, Phillip
item Beaver, James - UNIV OF PUERTO RICO
item Riley, Ronald - ROGERS SEED CO., IDAHO

Submitted to: Journal of the American Society for Horticultural Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 4, 1996
Publication Date: N/A

Interpretive Summary: Fresh market snap beans represent an estimated $80 million industry to Florida and other states in the southeastern U.S. This crop has recently come under attack by bean golden mosaic virus (BGMV). This virus represents a devasting disease that will completely wipe out production areas if appropriate disease control steps are not taken. We advocate the use of genetic resistance as the most economical control practice against this pathogen. We developed a genetic marker linked to an important source of resistance (a gene). This marker enables rapid selection of the resistance factor and thus development of resistant snap bean varieties for the southeastern U.S. fresh market production areas. These new virus resistant varieties once developed will have the potential for saving growers and processors millions of dollars in lost production.

Technical Abstract: Bean golden mosaic virus (BGMV) is a devastating disease of common bean (Phaseolus vulgaris L.) in tropical America. The most widely deployed source of resistance to BGMV is a recessive gene derived from the dry bean landrace cultivar `Garrapato' (Mexico) that conditions a `non-mosaic' partial resistance response to the pathogen. To expedite introgression of this resistance into snap bean for southern Florida and other susceptible dry bean market classes for the Caribbean and Central American regions, we sought a random amplified polymorphic DNA (RAPD) marker tightly linked to the gene. Two contrasting DNA bulks, one composed of five BGMV resistant and the other five susceptible F6 recombinant inbred lines, were used to screen for polymorphic fragments amplified by 300 decamer primers in the polymerase chain reaction. RAPDs generated between the bulks were analyzed across F2 populations segregating for both the marker and the gene. One codominant RAPD, XX570/530, was found to be tightly linked to the resistance gene locus with no recombinants among 91 dry bean F2 progeny and two recombinants (4.2 cM) among 48 snap bean F2 progeny. The codominant marker has utility for rapidly introgressing partial resistance to BGMV into susceptible germplasm.

Last Modified: 8/21/2014
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