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United States Department of Agriculture

Agricultural Research Service

Title: Optimization of Silver-Staining Aflp Technique for Soybean

Authors
item Vantoai, Tara
item Peng, Jiqing - THE OHIO STATE UNIVERSITY
item St Martin, Steve - THE OHIO STATE UNIVERSITY

Submitted to: Soybean Genetics Newsletter
Publication Type: Research Notes
Publication Acceptance Date: April 5, 1996
Publication Date: N/A

Technical Abstract: The AFLP technique is a very efficient DNA marker technique. Using a single primer pair, we were able to identify 14 polymorphic bands among two adapted and four plant introduction strains. Since Taq polymerase is expensive, the high number of polymorphism detected makes this technique more cost efficient than the RAPD technique. The technique is sensitive to the reproducibility of the thermoprofile within and across experiments. Silver-staining AFLP, while eliminating the use of radioactive isotopes, requires more skilled labor than the 32P or 33P-AFLP technique, and certainly more skilled labor than the RAPD technique.

Last Modified: 10/21/2014
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