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United States Department of Agriculture

Agricultural Research Service

Title: Detection and Biochemical Characterization of Insectide Resistance in the German Cockroach (Dictyoptera: Blattellidae)

Authors
item Valles, Steven
item Yu, Simon - UNIV. OF FLORIDA

Submitted to: Journal of Economic Entomology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 28, 1995
Publication Date: N/A

Interpretive Summary: A strain of German cockroach was collected from an institutional kitchen and cafeteria where efforts to control this population had been failing. We examined the strain to determine whether or not insecticide resistance was responsible for the control failures and if resistant, to determine the mechanisms responsible. Indeed, the strain was highly resistant to insecticides in several classes. Two key mechanisms were identified as being responsible for the resistance, enhanced detoxification and target site insensitivity. We showed that the use of insecticide synergists such as piperonyl butoxide could overcome the insecticide resistance.

Technical Abstract: Methods of microsome preparation were evaluated to determine their influence on the activity of cytochrome P450 monooxygenases in adult males and final instar nymphs of the German cockroach, Blattella germanica (L.). Aldrin epoxidase activity was significantly improved when homogenization occurred in a protected buffer and when alimentary canal contents were removed prior to homogenization. Apparently, a potent inhibitor of cytochrome P450 monooxygenases, but not of cytochrome c reductase, is present in the gut contents of German cockroaches. Aldrin epoxidase and methoxyresorufin (MR) O-demethyla midgut, hindgut, Malpighian tubules, fat body, and the remaining tissues of the carcass) from adult males and final instar nymphs. Adult male aldrin epoxidase activity was highest in midgut and Malpighian tubules, while no single tissue dominated in MR O-demethylase ac cspecific activity was 9- to 11-fold higher in midgut than in fat body, while MR O-demethylase specific activity was 17- to 22-fold higher in fat body than in midgut. In the final instar, aldrin epoxidase, MR O-demethylase, ethoxyresorufin (ER) O-deethylase, and cytochrome c reductase activities were lowest at the time of ecdysis and highest near the middle of the stadium. Dietary phenobarbital at the 0.2% level induced various microsomal oxidases including aldrin epoxidase, MR O-demethylase, ER O-deethylase, and cytochrome c reductase in final instar nymphs and adults.

Last Modified: 7/30/2014
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