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United States Department of Agriculture

Agricultural Research Service

Title: Biological and Molecular Diversity Within the Rmv Strain of Barley Yellow Dwarf Virus

item Lucio-Zavaleta, Eglantina - CORNELL UNIVERSITY
item Gray, Stewart

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: October 19, 1995
Publication Date: N/A

Technical Abstract: Recent collections of alate Rhopalosiphum padi in NY indicated that a significant number were transmitting the RMV strain of barley yellow dwarf virus (BYDV); a strain normally vectored by R. maidis, but not R. padi. Studies were then initiated to investigate the diversity of RMV isolates and the ability of field collected R. padi biotypes to vector RMV. Sixteen RMV isolates were obtained from viruliferous aphids, two transmitted by R. maidis and 14 transmitted by R. padi. All tested positive for RMV in ELISA. Reverse transcription-polymerase chain reaction (RT-PCR) amplification of the coat protein gene and subsequent restriction enzyme digestion identified six unique RFLP patterns. Four field isolates could not be amplified by RT-PCR using the same primer. R. maidis transmitted all the isolates, a laboratory maintained biotype of R. paid did not transmit any, and field-collected R. padi transmitted isolates from one RFLP group and two isolates not amplified by RT-PCR. The data confirm variability among RMV isolates both in terms of virus characteristics and vector associations.

Last Modified: 4/20/2015
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