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United States Department of Agriculture

Agricultural Research Service

Title: Combined Q-Banding and Fluorescence in Situ Hybridization for the Identification of Bovine Chromosomes 1 to 7

Authors
item Solinas-Toldo, S - ETH, ZURICH SWITZERLAND
item Mezzelani, A - ETH, ZURICH; CNR, MILANO
item Hawkins, Greg - AM. BREEDERS SERVICE
item Bishop, Michael - AM. BREEDERS SERVICE
item Olsaker, I - NORWEGIAN COLL VET MED
item Mackinlay, A - UNIV NEW SOUTH WALES
item Ferretti, L - CNR, MILANO ITALY
item Fries, Ruedi - ETH, ZURICH SWITZERLAND

Submitted to: Cytogenetics and Cell Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 22, 1994
Publication Date: N/A

Interpretive Summary: Maps of highly polymorphic markers are prerequisite for the systematic mapping and further analysis of economically important segregating loci, so-called economic trait loci (ETLs). Knowledge of the physical location of markers on the chromosomes is important for the efficient completion of the marker maps and will also be the basis for targeting the molecular analysis of ETLs to specific chromosome regions using, for example, chromosome microdissection. Physical mapping depends on a sufficiently detailed cytogenetic description and standardization, enabling unequivocal identification of the chromosomes.

Technical Abstract: Eleven probes were assigned to bovine chromosomes 1 to 7 by fluorescence in situ hybridization (FISH). The identification of chromosomes was based on QFQ-banding prior to in situ hybridization and comparison with the Reading Conference (1976) and ISCNDA (1989) standards. The probes used for FISH can now be utilized as identification and discrimination feature for bovine chromosomes 1 to 7 and particularly for chromosomes 4 and 6, which are difficult to distinguish. Comparison of our mapping data with previous assignment and of the standard chromosome banding patterns prompt us to propose a change in the ISCNDA nomenclature: ISCNDA chromosome 4 should be named chromosome 6 and vice versa. Chromosome 4 is marked by the ribosomal RNA cluster RNR3, and chromosome 6 is characterized by the casein gene cluster and an anonymous satellite (D6Z1).

Last Modified: 9/29/2014
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