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United States Department of Agriculture

Agricultural Research Service

Title: Administration of 2-Deoxy-D-Glucose in Swine: a Potential Stress Inducer

Author
item Stabel, Thomas

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: November 14, 1995
Publication Date: N/A

Technical Abstract: Stress is a broad term which has been used to identify a range of situations which alter an animal's homeostasis. Central to the study of porcine stress and its effect on immunity has been the need for a well defined stress inducer capable of simulating a physiological stress response. A metabolic stressor in rodents, 2-deoxy-D-glucose (2DG) produces an acute intracellular glucoprivation with all the physiological hallmarks of a physical stressor. Prior to a complete study of the physiological effects of 2DG in pigs it was necessary to determine an appropriate route and dose for 2DG administration. Pigs were cannulated in the femoral artery to allow for frequent blood collection with minimal external stress. The concentration and duration of 2DG in the blood was monitored while varying dose (500 or 750 mg/kg body weight) and route [intravenous (IV), subcutaneous (SC), intramuscular (IM), or intraperitoneal (IP)] of 2DG injection. SC injection of 2DG at either dos induced similar levels of blood 2DG (approximately 30 mg/dl) at 40 to 120 min postinjection, but no change in endogenous glucose concentrations was observed. IV injection of 2DG at either dose induced a similar increase in blood 2DG (approximately 60 mg/dl) at 10 to 90 min postinjection, with a concomitant increase in endogenous glucose. IP injection of 2DG induced a small increase in blood 2DG (less than or equal to 20 mg/dl) at 20 to 120 min postinjection, with a small increase in endogenous glucose. Detectable levels of 2DG were not found in the blood following IM injection and endogenous glucose levels remained baseline. Results indicate that 2DG can be detected in porcine blood for up to 2 h postinjection at levels that may be effective for induction of stress.

Last Modified: 9/10/2014
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