Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: November 14, 1995
Publication Date: N/A
Technical Abstract: Noncytopathic and cytopathic bovine viral diarrhea viruses were coisolated from two separate outbreaks of mucosal disease in cattle. Disease occurred within three weeks of administration of the same modified-live virus vaccine. Noncytopathic/cytopathic viral pairs coisolated from tissues from one animal in each outbreak were biologically cloned at limiting dilution. Each virus was analyzed by monoclonal antibody binding (gp53 specific), and by nucleic acid sequencing of select genomic regions. Noncytopathic viruses isolated from each outbreak were antigenically and genetically dissimilar to each other, and to their respective cytopathic viral pair. This indicated that cytopathic virus did not arise from noncytopathic virus, as would be expected in naturally occurring spontaneous mucosal disease. Cytopathic viruses from each outbreak were antigenically and genetically identical with each other, and with the cytopathic virus used to produce the vaccine. The history of each disease outbreak, and the laboratory findings, indicated postvaccinal mucosal disease was induced by disparate noncytopathic/cytopathic viral pairs.