|Qian, Zheng - CASE WESTERN RESERVE|
|Kung, Hsing-Jien - CASE WESTERN RESERVE|
Submitted to: Herpesvirus International Workshop
Publication Type: Abstract Only
Publication Acceptance Date: July 25, 1993
Publication Date: N/A
Technical Abstract: The chicken herpes virus Marek's Disease Virus (MDV) encodes a putative oncogen, meq, in its short repeat region. This protein has a bZIP motif, followed by a long stretch of proline-rich sequence at the C-terminal. This proline-rich domain includes two and half repeat sequences, each repeat of about 40 amino acids. When fused to Ga14 (1-147) DNA-binding motif, this domain can transactivate transcription from the Ga14 binding sites about 10 fold. Deletion analysis revealed that the C-terminal 30 amino acids are required for this function. Interestingly, the fusion protein with the repeat(s) alone can repress the transactivation to below the basal level. The presence of multiple transcripts suggests a regulating function by different domains. The transactivation by the native form of meq is being investigated.