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United States Department of Agriculture

Agricultural Research Service

Title: Segregation and Recombination of Solanum Brevidens Synteny Groups in Progeny of Somatic Hybrids with S. Tuberosum: Intra-Genomic Equals Or Exceeds Inter-Genomic Recombination

Authors
item Mcgrath, Mitchell - UNIVERSITY OF WISCONSIN
item Wielgus, Susan - UNIVERSITY OF WISCONSIN
item Helgeson, John

Submitted to: Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 26, 1995
Publication Date: N/A

Interpretive Summary: We have produced somatic hybrids between potato and various wild species related to potato including Solanum brevidens. In order to be useful for further plant improvement, it is necessary that the DNA from the wild species become incorporated into the chromosomes of potato. This study demonstrates that such recombination is possible. Thus, since these materials can be crossed with potato breeding lines, it will be possible to obtain new disease resistances to potato diseases and pests such as late blight, early blight, potato leaf roll virus and Erwinia soft rot. Eventually, it will be possible to grow the potato crop without pesticide application.

Technical Abstract: The Solanum brevidens genome was examined with 122 RAPD markers in a second backcross (BC2) population of 76 individuals derived from the sexual progeny of a fertile Solanum brevidens + S. tuberosum somatic hybrid backcrossed with S. tuberosum (potato). Each RAPD marker was assigned to one of 12 synteny groups that likely represent the n=12 chromosomes of S. brevidens. Synteny groups were either non-recombinant or recombinant. Non- recombinant synteny groups carried all assigned synteny group- specific RAPDs for an individual S. brevidens chromosome, and recombinant synteny groups had at least one, but not all, synteny group-specific RAPD markers. Nearly 40% of the S. brevidens synteny groups detected in this population were recombinant and all S. brevidens synteny groups (except chromosome 5) were involved in recombination. Recombination occurred within most intervals between markers. From BC3 segregation data as well as correlated BC2 data it was inferred that at least nine reassociations of S. brevidens synteny groups had occurred. Six of the nine changes involved translocation of S. brevidens markers between non-homologous S. brevidens chromosomes, and three S. brevidens markers were putatively introgressed into the potato genome.

Last Modified: 9/10/2014
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