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United States Department of Agriculture

Agricultural Research Service

Title: Chromosomal Organization of Oat Rflp Linkage Groups Using True and Immortal F1 Monosomic Series

item Rines, Howard
item Phillips, Ronald - UNIVERSITY OF MINNESOTA

Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: November 2, 1995
Publication Date: N/A

Technical Abstract: A genomic linkage map of oat (Avena sativa L.) constructed with more than 500 RFLP markers in a 'Kanota'/'Ogle' cross exceeds the expected 21 linkage groups, one for each chromosome of oat. Utilizing the Kanota oat monosomic series in crosses with Ogle, and the 'Sun II' oat monosomic series in crosses with Ogle and Kanota, DNA preparations from cytogenetically confirmed monosomic F1 plants and from bulked F2 plants used to mimic the monosomic F1 were screened with probes obtained from the oat RFLP map. Positive associations between probe binding sites and chromosomes were made only when the maternal allele of a polymorphic locus was absent in the true or immortal monosomic F1. Linkage groups could be associated with specific chromosomes using this method. In some cases, genetically unlinked loci were shown to be physically associated on the same chromosome. Heterogeneity and duplications among lines within and between Kanota and Sun II monosomic series could be detected. The monosomic F1 series represents a unique group of genetic stocks useful for integrating the physical and genetic maps of oat.

Last Modified: 4/22/2015
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