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United States Department of Agriculture

Agricultural Research Service

Title: Use of Genomic DNA Markers to Increase Linkage Map Coverage in Oat

item Gulbranson, Lisa - UNIVERSITY OF MINNESOTA
item Kianian, Shahryar - UNIVERSITY OF MINNESOTA
item Livingston, Suzanne - UNIVERSITY OF MINNESOTA
item O'Donoughue, Louise - AGRICULTURE CANADA
item Phillips, Ronald - UNIVERSITY OF MINNESOTA
item Rines, Howard

Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: November 2, 1995
Publication Date: N/A

Technical Abstract: An RFLP genetic map of oat containing over 600 markers was developed using 71 F6:7 recombinant inbred lines derived from the cross of A. byzantina cv. Kanota and A. sativa cv. Ogle. The genetic map was constructed predominantly through the use of cDNA probes. These markers produced 38 linkage groups as opposed to the expected 21, based upon the chromosome number of oat. Since genomic markers may provide greater coverage of the genome, probes from a Pst I oat genomic DNA library have principally been used in an attempt to cover gaps in the cDNA map. To date, eighty-two (82) markers show a tendency to map at or near the ends of linkage groups. Of nineteen current linkage groups where genomic sequences have been mapped, a genomic marker was the most terminal in twelve groups. In five of those twelve linkage groups, an additional genomic marker mapped adjacent to the terminal genomic marker, resulting in two genomic markers at the end of the elinkage group. In addition, genomic markers have been successful in showing linkage to previously unlinked cDNA markers.

Last Modified: 4/22/2015
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