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ARS Home » Plains Area » Bushland, Texas » Conservation and Production Research Laboratory » Livestock Nutrient Management Research » Research » Publications at this Location » Publication #60857
Title: EXTRACELLULAR NEURAMINIDASE PRODUCTION BY PASTEURELLA SPECIES ISOLATED FROMINFECTED ANIMALS

Author
item STRAUS, DAVID - TEXAS TECH UNIV., LUBBOCK
item Purdy, Charles

Submitted to: Current Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/22/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary: There is little data concerning the association of neuraminidase enzyme with various Pasteurella species and no data exist on what influence neuraminidase may have on the infectious process by the various species. We tested 721 field isolates of Pasteurella species (haemolytica, multocida, and tesudensis) from various animal species for the presence of neuraminidase. All of these Pasteurella strains were determined to have extracellular neuraminidase production. The data revealed the following: 1) Repeated passage of P. haemolytica on blood agar plates does not cause a decrease in enzyme production; 2) P. haemolytica serotypes 2 and 6 produce more neuraminidase than P. haemolytica serotype 1, P. multocida and P. tesudensis isolates; 3) There was no apparent change in neuraminidase production by P. haemolytica serotypes 1 and 2 isolates obtained from the same animal taken on different days in the feedyard; and 4) There was no significant change in neuraminidase production by P. haemolytica serotype isolates taken from the same animal at the auction market and later at the feedyard. Our data concerning extracellular neuraminidase production by several pathogenic Pasteurella species are important to the scientific community because it establishes a data base on 721 field isolates and the information obtained may lead to a better understanding of the Pasteurella disease process.

Technical Abstract: A total of 721 field isolates of various Pasteurella species (haemolytica, multocida, and tesudensis) from various regions of the United States were examined for extracellular neuraminidase production. All strains were grown and tested in the same way. Included were 372 P. haemolytica serotype 1 isolates, 181 P. haemolytica serotype 2 isolates, 63 P. haemolytica serotype 6 isolates, 101 Pasteurella multocida isolates and 4 Pasteurella tesudensis isolates. All Pasteurella species examined produced the enzyme. The data revealed the following: 1) Passage of strains of P. haemolytica on blood agar for several transfers does not cause a decrease in enzyme production; 2) P. haemolytica serotypes 2 and 6 produce more neuraminidase than P. haemolytica serotype 1, P. multocida, and P. tesudensis isolates; 3) There was no apparent change in neuraminidase production by P. haemolytica serotypes 1 and 2 isolates obtained from the same animal taken on different days in the feedyard; and 4) There was no significant change in neuraminidase production by P. haemolytica serotype 2 isolates taken from the same animal at the auction market and later at the feedyard.