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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #60847
Title: CYTOLOGICAL AND MOLECULAR CHARACTERIZATION OF MAIZE CHROMOSOME ADDITION LINES

Author
item RIERA-LIZARAZU, OSCAR - UNIVERSITY OF MINNESOTA
item Rines, Howard
item PHILLIPS, RONALD - UNIVERSITY OF MINNESOTA

Submitted to: Stadler Genetics Symposium
Publication Type: Abstract Only
Publication Acceptance Date: 6/20/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Oat (Avena sativa L; 2n=6x=42) x maize (Zea mays L; 2n=2x=20) crosses principally result in the production of oat haploid plants (2n=3x=21) following complete elimination of maize chromosomes. However, about one third (30 of 90) of the plants recovered from oat x maize crosses were partial oat-maize hybrids with one to four maize chromosomes in addition to oa haploid set of 21 oat chromosomes. Maize RFLP probes were used to identify the maize chromosomes present in the partial oat maize hybrids. Of thirteen hybrids with one maize chromosome that were analyzed, three contained maize chromosome 2, one had maize chromosome 3, two had maize chromosome 4, one had maize chromosome 5, one had maize chromosome 6, two had maize chromosome 7, one had maize chromosome 8, and two had maize chromosome 9. Of six hybrids with two maize chromosomes, one had maize chromosomes 4 and 8, one had maize chromosomes 7 and 8, one had maize chromosomes 2 and 7, one had maize chromosomes 5 and 9, one had maize chromosomes 4 and 7, and one contained two copies of chromosome 4. One hybrid analyzed contained maize chromosomes 4, 5, 6, and 9. No hybrids with maize chromosomes 1 and 10 were observed. The maize chromosome addition lines of oat represent unique, exciting materials which may be used in gene/marker mapping efforts, maize chromosome manipulations, study of maize gene expression in oat, and transfer of maize DNA, genes, or active transposable elements to oat.