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Title: ISOZYME VARIATION IN LYCHEE (LITCHI CHINENSIS SONN.)

Author
item Zee, Francis
item ARADHYA, MALLI - UNIVERSITY OF HAWAII
item MANSHARDT, RICHARD - UNIVERSITY OF HAWAII

Submitted to: HortScience
Publication Type: Abstract Only
Publication Acceptance Date: 4/7/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: A genetic diversity analysis involving 49 lychee (Litchi chinensis Sonn.) accessions using eight enzyme systems encoding twelve loci (Idh-1, Idh-2, Mdh-2, Per-1, Pgi-2, Pgm-1, Pgm-2, Skdh, Tpi-1, Tpi-2, Ugpp-1 and Ugpp-2) revealed moderate to high levels of genetic varia- bility. Cluster analysis of the isozyme data from 40 genetically different accessions of the total 49 identified three groups at the 50% level of genetic similarity, the largest of which contained 32 of the 40 accessions distributed in three sub-groups. The groups includ- ing the three sub-groups differed in frequency and composition of alleles at different loci. Polymorphism was observed in 77% of the loci with an overall mean of 2.2 alleles per locus and an observed heterozygosity of 0.387. The unbiased genetic identities (I) between groups ranged from 0.809 to 0.937. Summing over all 11 polymorphic loci, 16% of gene diversity was due to differentiation between groups and 84% within groups. Comparison of isozyme fingerprints revealed that some accessions with identical names, particularly of 'No mai tsz', 'Kwai me' and 'Hak ip', possessed different isozyme genotypes, while other acces- sions with different names displayed identical isozyme genotypes. Iso- zyme fingerprinting will be useful in revealing and resolving questions of clonal identity, which are common in lychee germplasm collections.