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Title: A RESTRICTION ENZYME MAP OF THE SEX-LINKED LATE-FEATHERING LOCUS OF CHICKENS

Author
item IRAQI FUAD - 3635-20-00
item ROBINSON D - CASE WESTERN RESERVE UNIV
item Smith, Eugene

Submitted to: Poultry Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/20/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary: Although the sex-linked, late-feathering mutation is used extensively in gender selection among commercial production lines, the molecular nature of this important mutation remains unknown. The construction of this genetic map and molecular hybridization probes described herein will help future investigations on the molecular organization of this region and possibly identify the gene that confers late-feathering.

Technical Abstract: A 34 kilobase restriction endonuclease map of the region associated with an endogenous virus integration site and K, the gene that confers sex- linked late-feathering in chickens, is presented. Hybridizations of genomic blots of DNA from early and late-feathering White Leghorns indicated that the region also contains other different repetitive elements upstream from a chicken repetitive (CR1) element. With the construction of artificial chromosome libraries, this map and the probes described should be useful in future investigations aimed at identifying the molecular alterations associated with this mutation.