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ARS Home » Plains Area » Kerrville, Texas » Knipling-Bushland U.S. Livestock Insects Research Laboratory » LAPRU » Research » Publications at this Location » Publication #320508
Research Project: Genomics of Livestock Pests

Location: Livestock Arthropod Pests Research

Title: Optimization and validation of molecular diagnosis of pyrethroid pesticide resistance in Brazilian populations of horn flies

Author
item BRITO, LUCIANA GATTO - Embrapa
item OLIVEIRA, MARCIA CRISTINA - Embrapa
item BARBIERI, FABIO DA SILVA - Embrapa
item IBELLI, ADRIANA MERCIA - Embrapa
item DA SILVA, IVANETE - Institute Of Biology - Brazil
item DOS SANTOS, ANA PAULA LEITE - Institute Of Biology - Brazil
item DA SILVA, RENATA REIS - Embrapa
item Guerrero, Felicito

Submitted to: Technical Report
Publication Type: Experiment Station
Publication Acceptance Date: 6/1/2013
Publication Date: 6/1/2013
Citation: Brito, L., Oliveira, M., Barbieri, F., Ibelli, A., Da Silva, I., Dos Santos, A., Da Silva, R., Guerrero, F. 2013. Optimization and validation of molecular diagnosis of pyrethroid pesticide resistance in Brazilian populations of horn flies. Technical Report. 130:1-8.

Interpretive Summary: Pyrethroid resistance is a serious problem to cattle producers in Brazil. There are specific resistance-associated mutations in the region of DNA that codes for the target of pyrethroids, the sodium channel. The presence of these mutations causes target site resistance to pyrethroids. A DNA-based diagnostic assay developed by ARS researchers that detects these mutations in individual horn flies was validated on populations of horn flies in Brazil.

Technical Abstract: Pyrethroid resistance is a serious problem to cattle producers in Brazil. There are specific resistance-associated mutations, known as kdr and super-kdr, in the region of DNA that codes for the target of pyrethroids, the sodium channel protein. The presence of these mutations in the sodium channel causes target site resistance to pyrethroids, affecting the binding and activity of pyrethroids to sodium channels. A PCR-based multiplex diagnostic assay developed by ARS researchers that detects the kdr and super-kdr mutations in individual horn flies was validated on populations of horn flies in Brazil.