Skip to main content
ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Mycotoxin Prevention and Applied Microbiology Research » Research » Publications at this Location » Publication #312841
Title: Sequencing and molecular dissection of Sk-2 in Neurospora

Author
item HARVEY, AUSTIN - Illinois State University
item SVEDBERG, JESPER - Uppsala University
item REHARD, DAVID - University Of Missouri
item Brown, Daren
item WILSON, RICHARD - University Of Nebraska
item SHIU, PATRICK K - University Of Missouri
item JOHANNESSON, HANNA - Uppsala University
item HAMMOND, THOMAS - Illinois State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/22/2015
Publication Date: 3/22/2015
Citation: Harvey, A.M., Svedberg, J., Rehard, D.G., Brown, D.W., Wilson, R., Shiu, P.T., Johannesson, H., Hammond, T.M. 2015. Sequencing and molecular dissection of Sk-2 in Neurospora [abstract].

Interpretive Summary:

Technical Abstract: Neurospora Spore killer-2 is a selfish meiotic drive element that kills its non-Sk-2 siblings during sexual sporulation. Although the location of Sk-2 has been mapped to a large recombination-suppressed region of chromosome III, the physical length and exact borders of the element have remained undefined. We recently assembled a draft Sk-2 sequence suggesting it to be 2 Mb long with chromosome rearrangements that could account for recombination suppression. In addition, we identified a mutation that completely eliminates spore killing. The corresponding locus, rfk-1 (required for killing), was mapped to the right border of the Sk-2 element. Experiments to molecularly characterize rfk-1, test its function in other fungi, and elucidate the mechanism of spore killing are in progress.