Skip to main content
ARS Home » Southeast Area » Tifton, Georgia » Crop Protection and Management Research » Research » Publications at this Location » Publication #311818
Title: Genetic mapping and QTL analysis of disease resistance traits in peanut population Tifrunner x GT-C20

Author
item WANG, HUI - University Of Georgia
item KHERA, PAWAN - University Of Georgia
item CULBREATH, ALBERT - University Of Georgia
item PANDEY, MANISH - International Crops Research Institute For Semi-Arid Tropics (ICRISAT) - India
item VARSHNEY, RAJEEV - International Crops Research Institute For Semi-Arid Tropics (ICRISAT) - India
item WANG, XINGJUN - Genome Sequencing Center
item LIAO, BOSHOU - Genome Sequencing Center
item ZHANG, XINYOU - Genome Sequencing Center
item WAG, JIANPING - University Of Florida
item Holbrook, Carl - Corley
item Guo, Baozhu

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 11/8/2014
Publication Date: 11/10/2014
Citation: Wang, H., Khera, P., Culbreath, A.K., Pandey, M.K., Varshney, R.K., Wang, X., Liao, B., Zhang, X., Wag, J., Holbrook Jr, C.C., Guo, B. 2014. Genetic mapping and QTL analysis of disease resistance traits in peanut population Tifrunner x GT-C20. Meeting Abstract. Advanced in Arachis through Genomics and Biotechnology (AAGB) meeting, November, 10-14, 2014, Savannah, Georgia.

Interpretive Summary:

Technical Abstract: A genetic map of peanut (Arachis hypogaea L.) with 426 SSR markers was constructed using a population of 162 recombinant inbred lines (RILs) from a cross between ‘Tifrunner’ and ‘GT-C20’. Linkage groups (LGs) were assigned to chromosomes using published peanut reference maps. The total length of the map was 1980.78 cM with an average marker interval length of 4.6 cM. This map has the highest number of SSR markers for a single population. This genetic map was used to identify quantitative trait loci (QTL) for resistance to several diseases. These RILs were evaluated for early leaf spot (ELS), caused by Cercospora arachidicola, late leaf spot (LLS), caused by Cercosporidium personatum, and Tomato spotted wilt virus (TSWV) in the field from 2009 to 2013. QTL analysis resulted in the identification of a total of 47 QTLs on 16 LGs with phenotypic variance (PV) ranging from 6.26% to 15.54%, and 13 major QTLs (PV >10%) were identified including 14 (7 major) on 10 LGs, 22 (5 major) on 8 LGs, and 11 (1 major) on 7 LGs for ELS, LLS, and TSWV, respectively. Four consistent QTL regions expressed in more than two environments were also identified for ELS (LG5, LG6), LLS (LG5) and TSWV (LG4) with PV of up to 13.20%, 12.35% and 14.40%, respectively. Interestingly, LG5 was identified as a “resistance gene rich” for both ELS and LLS. Furthermore, allelic contribution of resistance and susceptibility was calculated and alleles with higher resistance score were identified. Further study is needed to identify the potential resistance gene(s) through fine-mapping approaches.