Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: February 27, 2014
Publication Date: N/A
Technical Abstract: Foodborne pathogens can cause various diseases and even death when humans consume foods contaminated with microbial pathogens. Traditional culture-based direct plating methods are still the “gold standard” for presumptive-positive pathogen screening. Although considerable research has been devoted to development and use of biochemical, serological, and molecular methods for confirmation of presumptive-positive colonies, such as latex agglutination and polymerase chain reaction (PCR), little research has been done to develop methods and techniques for non-invasive optical screening of presumptive-positive colonies, while keeping them on agar-filled Petri dishes. Hyperspectral imaging is a non-destructive and non-contact optical imaging technique that combines aspects of conventional imaging and vibrational spectroscopy so that data can provide two-dimensional spatial information on colony shapes and spectral information at every pixel in each colony under test. The spectral “fingerprints” of bacteria provided by hyperspectral imaging can be used for detection and identification of pathogens. This book chapter covers the potential and efficacy of hyperspectral imaging for detection and identification of pathogenic colonies such as Campylobacter and Shiga-toxin producing Escherichia coli (STEC).