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United States Department of Agriculture

Agricultural Research Service

Research Project: Conservation, Characterization, and Evaluation of Plant Genetic Resources and Associated Information

Location: Plant Genetic Resources Conservation Unit

Title: Recovery and purification of spanish high oleate peanut ‘AT-9899’

Authors
item Chen, Zhenbang -
item Wang, Ming
item Lamb, Marshall
item Dang, Phat
item Bostick, J -
item Chen, Charles -

Submitted to: American Peanut Research and Education Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: March 30, 2014
Publication Date: July 9, 2014
Citation: Chen, Z., Wang, M.L., Lamb, M.C., Dang, P.M., Bostick, J., Chen, C.Y. 2014. Recovery and purification of spanish high oleate peanut ‘AT-9899’. [abstract] American Peanut Research and Education Society Abstracts. Paper No. 35.

Interpretive Summary: A Spanish market type peanut “AT-9899” was developed by Golden Peanut Company in 2002. It has spreading growth habit and mid maturity. Due to high level of oleate and small seed size, it is grown specifically for confectionery market in the USA and Mexico. However, from the time of development and release until 2010 the level of the high oleic trait had diminished, either due to impurity at release or contamination after release, to the point that the variety was not meeting industry requirements to be classified as high oleic. In order to recover and purify ‘AT-9899’, 1,600 individual plants were initially selected based on phenotype in field in 2010. After shelling, 600 plants were further evaluated by GC analysis and SNP marker-assisted evaluation, and 300 plants were identified as the most similar to original ‘AT-9899’. The 300-plant seeds were planted as breeder seeds for seed increase and 3,200 pounds of the seeds were harvested in 2011. In 2013, about 90 tons of foundation seeds have been successfully achieved. In the meantime, through AFLP profile, potential mixers were identified. The result indicated that marker-assisted selection not only can improve the efficiency of breeding program but also can be used in seed industry for recovery and security of seed purity.

Technical Abstract: “AT-9899” developed in Golden Peanut Company in 2002 is a Spanish market type peanut. It has spreading growth habit and mid maturity. Due to high level of oleate and small seed size, it is grown specifically for confectionery market in the USA and Mexico. However, from the time of development and release until 2010 the level of the high oleic trait had diminished, either due to impurity at release or contamination after release, to the point that the variety was not meeting industry requirements to be classified as high oleic. In order to recover and purify ‘AT-9899’, 1,600 individual plants were initially selected based on phenotype in field in 2010. After shelling, 600 plants were further evaluated by GC analysis and SNP marker-assisted evaluation, and 300 plants were identified as the most similar to original ‘AT-9899’. The 300-plant seeds were planted as breeder seeds for seed increase and 3,200 pounds of the seeds were harvested in 2011. In 2013, about 90 tons of foundation seeds have been successfully achieved. In the meantime, through AFLP profile, potential mixers were identified. The result indicated that marker-assisted selection not only can improve the efficiency of breeding program but also can be used in seed industry for recovery and security of seed purity.

Last Modified: 10/25/2014
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