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United States Department of Agriculture

Agricultural Research Service

Research Project: Genetic Enhancement of Sorghum as a Versatile Crop

Location: Plant Stress and Germplasm Development Research

Title: Proteome profiling of seed from inbred and mutant line of sorghum (Sorghum bicolor)

Authors
item Swapan, Roy -
item Abu, Kamal -
item Hye-Rim, Kim -
item Soo-Jeong, Kwon -
item Hee-Young, Jang -
item Jung-Hee, Ko -
item Jong-In, Kim -
item Tae-Seok, Ko -
item XIN, ZHANGUO
item Sun-Hee, Woo -

Submitted to: Australian Journal of Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: May 1, 2014
Publication Date: July 24, 2014
Citation: Swapan, R., Abu, K., Hye-Rim, K., Soo-Jeong, K., Hee-Young, J., Jung-Hee, K., Jong-In, K., Tae-Seok, K., Xin, Z., Sun-Hee, W. 2014. Proteome profiling of seed from inbred and mutant line of sorghum (Sorghum bicolor). Australian Journal of Crop Science. 8(4):606-611.

Interpretive Summary: Grain sorghum is a major staple food, with fifth rank among the cereals world-wide, considering its importance for food and feed applications. Enhancing sorghum nutrition value is an important goal of sorghum improvement. In this study, we executed a comprehensive proteomic study to investigate the seed storage proteins using mature seeds from inbred line (BTx623) and mutant line (M2P1207) of sorghum. Proteins were separated from the mature seeds using IEF in the first- dimension and 2D-PAGE in the second dimension along with MALDI-TOF-TOF mass spectrometry. Two dimensional gels stained with coomassie brilliant blue (CBB), we confirmed 62 differential expressed proteins (= 2-fold) out of 293 protein spots using image analysis by Progenesis SameSpot software. Out of total differential expressed spots, 34 differential expressed protein spots (= 2-fold) were analyzed by mass spectrometry. Out of 34 protein spots, we identified 19 protein spots as up-regulated whereas 15 protein spots as down-regulated. The identified seed storage proteins can economically be used to assess genetic variation and relation in sorghum germplasm.

Technical Abstract: Grain sorghum is a major staple food, with fifth rank among the cereals world-wide, considering its importance for food and feed applications. Cereals are main part of human nutrition and strategic resources. In this study, we executed a comprehensive proteomic study to investigate the seed storage proteins using mature seeds from inbred line (BTx623) and mutant line (M2P1207) of sorghum. Proteins were separated from the mature seeds using IEF in the first- dimension and 2D-PAGE in the second dimension along with MALDI-TOF-TOF mass spectrometry. Two dimensional gels stained with coomassie brilliant blue (CBB), we confirmed 62 differential expressed proteins (= 2-fold) out of 293 protein spots using image analysis by Progenesis SameSpot software. Out of total differential expressed spots, 34 differential expressed protein spots (= 2-fold) were analyzed by mass spectrometry. Out of 34 protein spots, we identified 19 protein spots as up-regulated whereas 15 protein spots as down-regulated. The identified seed storage proteins can economically be used to assess genetic variation and relation in sorghum germplasm.

Last Modified: 9/29/2014
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