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United States Department of Agriculture

Agricultural Research Service

Research Project: BITING ARTHROPODS: INTEGRATED PEST MANAGEMENT

Location: Mosquito and Fly Research Unit

Title: Green synthesis of silver nanoparticles using Calotropis gigantea and their potential mosquito larvicidal property

Authors
item Priya, S. -
item Murugan, K. -
item Priya, A. -
item Dinesh, D. -
item Panneerselvam, C. -
item Durga Devi, G. -
item Chandramohan, B. -
item Mahesh Kumar, P. -
item Barnard, Donald
item Xue, Rui-De -
item Hwang, Jiang-Shiou -
item Nicoletti, M. -
item Chandrasekar, R. -
item Amsath, A. -
item Bhagooli, R. -
item Wei, Hui -

Submitted to: Journal of Pure and Applied Zoology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: May 24, 2014
Publication Date: May 27, 2014
Citation: Priya, S., Murugan, K., Priya, A., Dinesh, D., Panneerselvam, C., Durga Devi, G., Chandramohan, B., Mahesh Kumar, P., Barnard, D.R., Xue, R., Hwang, J., Nicoletti, M., Chandrasekar, R., Amsath, A., Bhagooli, R., Wei, H. 2014. Green synthesis of silver nanoparticles using Calotropis gigantea and their potential mosquito larvicidal property. Journal of Pure and Applied Zoology. 2(2):128-137.

Interpretive Summary: Nanoparticles synthesized from plant extract (in the presence of silver nitrate) can be toxic to mosquito larvae and pupae. In this study, a team of Indian and ARS scientists synthesized silver nanoparticles from the leaf extract of dogbane and tested the nanoparticles for toxicity to larvae of the Yellow Fever mosquito, an important vector of human malaria (Anopheles stephensi). The nanoparticles were characterized using a variety of light-based and microscopic analytical methods. Against mosquito larvae, silver nanoparticles caused more than 90% mortality when used at a concentration of 15-17 parts per million for 24 h. The results of the study showed that silver nanoparticles, which are comparatively easy to produce, have significant potential for the control of disease-transmitting mosquitoes.

Technical Abstract: In recent years the utilization of secondary metabolites from plant extract has emerged as a novel technology for the synthesis of nanoparticles. The aim of the present study was to evaluate the effect of plant synthesized silver nanoparticles (Ag NPs) using aqueous leaf extract of Calotropis gigantea to control the dengue vector Aedes aegypti and the malaria vector, Anopheles stephensi. Synthesized AgNPs were characterized by UV–vis spectrum, scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), and Fourier transform infrared spectroscopy (FTIR). Synthesized silver nanoparticles (AgNPs) were confirmed by analyzing the excitation of surface plasmon resonance (SPR) using UV–vis spectrophotometer at 410 nm. SEM analysis of the synthesized Ag NPs showed clustered and irregular shapes, mostly aggregated, 20–35 nm in size. The chemical composition of elements present in the solution was determined by energy dispersive spectrum. FTIR analysis of nanoparticles indicated the presence of proteins. Biosynthesis of nanoparticles is triggered by several compounds and reducing agents present in the biological extract. The results suggest that the synthesized Ag NPs may be an eco-friendly approach to the control of immature Ae. aegypti and An. stephensi.

Last Modified: 10/21/2014
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