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ARS Home » Northeast Area » Boston, Massachusetts » Jean Mayer Human Nutrition Research Center On Aging » Research » Publications at this Location » Publication #307238
Title: Identification of serum analytes and metabolites associated with aerobic capacity

Author
item LUSTGARTEN, MICHAEL - Jean Mayer Human Nutrition Research Center On Aging At Tufts University
item PRICE, LORI LYN - Tufts - New England Medical Center
item LOGVINENKO, TANYA - Tufts - New England Medical Center
item HATZIS, CHRISTOS - Nuvera Biosciences, Inc
item PADUKONE, NANDAN - Nuvera Biosciences, Inc
item REO, NICHOLAS - Wright State University
item PHILLIPS, EDWARD - Jean Mayer Human Nutrition Research Center On Aging At Tufts University
item KIRN, DYLAN - Jean Mayer Human Nutrition Research Center On Aging At Tufts University
item MILLS, JOHN - Jean Mayer Human Nutrition Research Center On Aging At Tufts University
item FIELDING, ROGER - Jean Mayer Human Nutrition Research Center On Aging At Tufts University

Submitted to: European Journal of Applied Physiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/15/2012
Publication Date: 5/1/2013
Citation: Lustgarten, M.S., Price, L., Logvinenko, T., Hatzis, C., Padukone, N., Reo, N.V., Phillips, E.M., Kirn, D., Mills, J., Fielding, R.A. 2013. Identification of serum analytes and metabolites associated with aerobic capacity. European Journal of Applied Physiology. 113(5):1311-1320.

Interpretive Summary: The goal of the present study was to identify enzymes, electrolytes or metabolites present in blood that were significantly associated with cardiovascular fitness. Using 2 separate methods (chemistry screen and mass spectrometry) of measuring substance found in blood obtained from 77 young subjects (18-35 y), we discovered significant associations between 2 enzymes and 15 metabolites with cardiovascular fitness. Statistical analysis identified a muscle/liver enzyme and a Vitamin B6 metabolite as overall biomarkers of cardiovascular fitness, in separate models. In conclusion, we identified for the first time in a young, healthy cohort blood substances containing significant associations with cardiovascular fitness.

Technical Abstract: Studies aimed at identifying serum markers of cellular metabolism (biomarkers) that are associated at baseline with aerobic capacity (V02 max) in young, healthy individuals have yet to be reported. Therefore, the goal of the present study was to use the standard chemistry screen and untargeted mass spectrometry (MS)-based metabolomic profiling to identify significant associations between baseline levels of serum analytes or metabolites with V02 max (77 subjects, age range 18-35y). Use of multivariable linear regression identified three analytes (standard chemistry screen) and twenty-three metabolites (MS-based metabolomics) containing significant, sex-adjusted associations with V02 max. In addition, fourteen metabolites were found to contain sex-specific associations with aerobic capacity. Subsequent stepwise multivariable linear regression identified the combination of SGOT, 4-ethylphenylsulfate, tryptophan, ‘y-tocopherol, and a-hydroxyisovalerate as overall, sex-adjusted baseline predictors of V02 max (adjusted R2 = 0.66). However, the results of the stepwise model were found to be sensitive to outliers; therefore, random forest (RF) regression was performed. Use of RF regression identified a combination of seven covariates that explained 57.6% of the variability inherent in V02 max. Furthermore, inclusion of significant analytes, metabolites and sex-specific metabolites into a stepwise regression model identified the combination of five metabolites in males and seven metabolites in females as being able to explain 80% and 58% of the variability inherent in V02 max, respectively. In conclusion, the evidence presented in the current report is the first attempt to identify baseline serum biomarkers that are significantly associated with V02 max in young, healthy adult humans.