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Title: Quantitative determination of seven chemical constituents and chemo-type differentiation of chamomiles using high-performance thin-layer chromatography

Author
item SAGI, SATYANARAYANARA - University Of Mississippi
item AVULA, BHARATHI - University Of Mississippi
item WANG, YAN-HONG - University Of Mississippi
item ZHAO, JIANPING - University Of Mississippi
item KHAN, IKHLAS - University Of Mississippi

Submitted to: Journal of Separation Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/15/2014
Publication Date: 9/1/2014
Publication URL: https://handle.nal.usda.gov/10113/62280
Citation: Sagi, S.J., Avula, B., Wang, Y., Zhao, J., Khan, I.A. 2014. Quantitative determination of seven chemical constituents and chemo-type differentiation of chamomiles using high-performance thin-layer chromatography. Journal of Separation Science. 37(19):2797-2804.

Interpretive Summary: One of the most commonly consumed single ingredient herbal teas is chamomile, prepared with dried flowers from Matricaria recutita L. The other two chamomiles Anthemis nobilis L. (Roman Chamomile) and Chrysanthemum morifolium Ramat were also used in these preparations. A simple HPTLC method was developed to differentiate the three chamomiles. This method is first report on Chamomile analysis using HPTLC. HPTLC method was simple and fast for analysis of large number of samples. It can analyze up to 20 samples in single run. 15 authenticated chamomile samples, 7 tea bags and 2 capsules were analyzed and found that chemical compounds varying in three types of chamomiles and dietary supplements. The developed HPTLC method can be used as an quality control tool for assessing the quality of chamomile raw material.

Technical Abstract: Matricaria recutita L. (German Chamomile), Anthemis nobilis L. (Roman Chamomile) and Chrysanthemum morifolium Ramat are commonly used chamomiles. High performance thin layer chromatographic (HPTLC) method was developed for estimation of six flavonoids (rutin, luteolin-7-O-ß-glucoside, chamaemeloside, apigenin-7-O-ß-glucoside, luteolin, apigenin) and one coumarin, umbelliferone from chamomile plant samples and dietary supplements. 15 authenticated chamomile samples and 9 dietary supplements were analyzed using developed HPTLC. The results showed the content of the chemical compounds are varying among the three types of chamomile samples.