Page Banner

United States Department of Agriculture

Agricultural Research Service

Research Project: National Animal Germplasm Program

Location: Plant And Animal Genetic Resources Preservation Research Unit

Title: Membrane modification strategies for cryopreservation. pp xx-xx. In: Willem F. Wolkers and Harriette Oldenhof (eds.). Methods in cryopreservation and freeze-drying. The lab protocol series Methods in Molecular Biology.

Authors
item Purdy, Phil
item Grahm, James -

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: May 21, 2014
Publication Date: N/A

Technical Abstract: Cell membranes can be modified using cyclodextrins loaded with lipids or unilamellar liposomes. Lipid choice can greatly influence the organization of the targeted membrane and result in a cell that is more capable of surviving cryopreservation due to altered membrane phase transition properties or membrane reorganization that may alter the normal physiologic processes of the treated cell. The protocols described here will explain the preparation of the cyclodextrins and liposomes, impact of the amount and type of lipids, and general principles for treating cells using either of these technologies.

Last Modified: 12/24/2014
Footer Content Back to Top of Page