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United States Department of Agriculture

Agricultural Research Service

Research Project: CEREAL RUST FUNGI: GENETICS, POPULATION BIOLOGY, AND HOST-PATHOGEN INTERACTIONS

Location: Cereal Disease Laboratory

Title: A consensus map for Ug99 stem rust resistance loci in wheat

Authors
item Yu, Long Xi
item Hugues, Barbier -
item Rouse, Matthew
item Singh, Sukhwinder -
item Singh, Ravi -
item Bhavani, Sridhar -
item Huerta-Espino, Julio -
item Sorrells, Mark -

Submitted to: Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: May 5, 2014
Publication Date: June 6, 2014
Citation: Yu, L., Hugues, B., Rouse, M.N., Singh, S., Singh, R.P., Bhavani, S., Huerta-Espino, J., Sorrells, M.E. 2014. A Consensus Map for Ug99 Stem Rust Resistance Loci in Wheat. Theoretical and Applied Genetics. Available: http://link.springer.com/article/10.1007%2Fs00122-014-2326-7.

Interpretive Summary: Wheat stem rust, a disease caused by a fungus, can result in severe yield losses in wheat. In recent years epidemics of this disease, caused by the emerging Ug99 strain, have been reported. The global effort to identify new sources of resistance to Ug99 has resulted in numerous studies reporting effective plant resistance genes. The purpose of our study was to assemble all available information on wheat molecular markers associated with stem rust resistance from 21 recent studies. We constructed a stem rust resistance loci consensus wheat map incorporating molecular markers. Most of the markers associated with stem rust resistance have been identified in more than one population. Several resistance loci appeared to overlap with known Sr genes including Sr2, SrND643, Sr25 and Sr57 (Lr34), while other significant markers were located in chromosome regions where no Sr genes have been previously reported. This consensus map provides a comprehensive source of information for Ug99 stem rust resistance loci identification, and marker-assisted selection for breeding resistant wheat. This information will facilitate the breeding of Ug99 stem rust resistant wheat to protect United States wheat production.

Technical Abstract: The global effort to identify new sources of resistance to wheat stem rust, caused by Puccinia graminis f. sp. tritici race group Ug99, has resulted in numerous studies reporting both qualitative genes and quantitative trait loci (QTLs). The purpose of our study was to assemble all available information on loci associated with stem rust resistance from 21 recent studies on Triticum aestivum (bread wheat) and Triticum turgidum subsp. durum (durum wheat). The software LPmerge was used to construct a stem rust resistance loci consensus wheat map incorporating Single Nucleotide Polymorphism (SNP), Diversity Array Technology (DArT), Genotyping-by-Sequencing (GBS) as well as Simple Sequence Repeat (SSR) markers. Most of the markers associated with stem rust resistance have been identified in more than one population. Several resistance loci appeared to overlap with known Sr genes including Sr2, SrND643, Sr25 and Sr57 (Lr34), while other significant markers were located in chromosome regions where no Sr genes have been previously reported. This consensus map provides a comprehensive source of information for Ug99 stem rust resistance loci identification genome-wide and for selection of markers for marker-assisted selection for breeding resistant wheat.

Last Modified: 11/25/2014
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