Author
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Hinton Jr, Arthur |
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Submitted to: European Poultry Conference Proceedings
Publication Type: Abstract Only Publication Acceptance Date: 2/27/2014 Publication Date: 6/23/2014 Citation: Hinton Jr, A. 2014. Comparison of fumerate-pyruvate media and beef extract media for aerobically culturing Campylobacter species. European Poultry Conference Proceedings. Stavanger, Norway, June 23-27, 2014. Interpretive Summary: None. Technical Abstract: Media supplemented with fumarate, pyruvate, and a vitamin-mineral solution or with beef extract were compared for the ability to support aerobic growth of Campylobacter. Basal broth composed of tryptose, yeast extract, bicarbonate, and agar was supplemented with 30 mM fumarate, 100 mM pyruvate, and a vitamin-mineral solution (FPVM) or with 5% beef extract (BE). Media were inoculated with Campylobacter coli, Campylobacter fetus, Campylobacter lari, Campylobacter jejuni 2b, or Campylobacter jejuni 33560. Inoculated media were placed in a Bioscreen Microbiology Reader and optical densities (OD) were measured during incubation for 72 h at 37C. Inoculated media was also incubated aerobically for 48 h at 37C in culture flasks, and cfu/ml were enumerated on selective Campylobacter agar. Results indicated that all isolates grew when incubated aerobically in either medium; however, the OD of C. jejuni 2b and C. fetus cultures in FPVM were significantly higher than OD of cultures in BE, while the OD of C. lari cultures were significantly higher in BE. Furthermore, significantly more cfu/ml of C. lari were recovered from BE than from FPVM; however, there was no significant difference in the number other isolates recovered from either medium. Findings indicate that FPVM or BE media can be used to culture Campylobacter aerobically, though only 1 of the media may support optimal growth of some isolates. Use of these media may provide an alternative for culturing this foodborne pathogen. |
