Page Banner

United States Department of Agriculture

Agricultural Research Service

Research Project: Improving Fruit Quality, Disease Resistance, and Tolerance to Abiotic Stress in Grape

Location: Grape Genetics Research

Title: 16d10 Sirnas Inhibit Root-Knot Nematode Infection in Transgenic Grape Hairy Roots

Authors
item YANG, YINGZHEN
item Jittayasothorn, Yingyos -
item Chronis, Demosthenis
item WANG, XIAOHONG
item Cousins, Peter -
item ZHONG, GAN-YUAN

Submitted to: Plant and Animal Genome
Publication Type: Abstract Only
Publication Acceptance Date: January 17, 2014
Publication Date: N/A

Technical Abstract: To develop a biotech-based solution for controlling Root-knot nematodes (RKNs) in grapes, we evaluated the efficacy of plant-derived RNA interference (RNAi) silencing of a conserved RKN effector gene, 16D10, for nematode resistance in transgenic grape hairy roots. Two hairpin-based silencing constructs, containing a stem sequence of 42 bp (pART27-42) or 271 bp (pART27-271) of the 16D10 gene, were transformed into grape hairy roots and compared for their small interfering RNA (siRNA) production and efficacy on suppression of nematode infection. Transgenic hairy root lines carrying either of the two RNAi constructs showed less susceptibility to nematode infection compared with control. Small RNA libraries from four pART27-42 and two pART27-271 hairy root lines were sequenced using an Illumina sequencing technology and several interesting molecular characteristics of 16D10-specific siRNAs from these libraries were observed. This study demonstrated the feasibility of using a plant-derived RNAi approach for generation of novel nematode resistance in grapes and provided insights into optimizing plant RNAi constructs for enhancing silencing efficacies.

Last Modified: 9/10/2014
Footer Content Back to Top of Page