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United States Department of Agriculture

Agricultural Research Service

Research Project: Intervention Strategies to Control and Prevent Disease Outbreaks Caused by Avian Influenza and Other Emerging Poultry Pathogens

Location: Exotic and Emerging Avian Viral Diseases Research Unit

Title: Avian influenza virus isolation, propagation and titration in embryonated chicken eggs

Authors
item Spackman, Erica
item Killian, Mary -

Submitted to: Animal Influenza Virus
Publication Type: Book / Chapter
Publication Acceptance Date: February 3, 2014
Publication Date: June 11, 2014
Citation: Spackman, E., Killian, M.L. 2014. Avian influenza virus isolation, propagation and titration in embryonated chicken eggs. In: Spackman, E., editor. Animal Influenza Virus: Methods in Molecular Biology. Volume 1161. London: Humana Press. p. 125-140.

Interpretive Summary: Avian influenza (AI) virus is usually isolated, propagated, and titrated in embryonated chickens eggs (ECE). Most any sample type can be accommodated for culture with appropriate processing. Isolation may also be accomplished in cell culture particularly if mammalian lineage isolates are suspected, for example, swine influenza in turkey specimens. Culture is highly sensitive, but is not specific for influenza A, which may be an advantage because a sample may be screened for several agents at once. Once an agent is isolated in culture the presence of AI virus is confirmed with any of several assays. The methods for propagating virus isolates in ECE are described.

Technical Abstract: Avian influenza (AI) virus is usually isolated, propagated, and titrated in embryonated chickens eggs (ECE). Most any sample type can be accommodated for culture with appropriate processing. Isolation may also be accomplished in cell culture particularly if mammalian lineage isolates are suspected, for example, swine influenza in turkey specimens. Culture is highly sensitive, but is not specific for influenza A, which may be an advantage because a sample may be screened for several agents at once. Once an agent is isolated in culture the presence of AI virus is confirmed with any of several assays. The methods for propagating virus isolates in ECE are described.

Last Modified: 7/23/2014
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