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United States Department of Agriculture

Agricultural Research Service

Research Project: Innovations that Improve the Efficiency and Effectiveness of Managing and Preserving Ex Situ Plant Germplasm Collections

Location: Plant Germplasm Preservation Research Unit

Title: Plant shoot tip response to treatment with plant vitrification solution 2

Authors
item Volk, Gayle
item Henk, Adam
item Bonnart, Remi
item Shepherd, Ashley -
item Gross, Briana -

Submitted to: Acta Horticulturae
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: November 15, 2013
Publication Date: June 22, 2014
Citation: Volk, G.M., Henk, A.D., Bonnart, R.M., Shepherd, A., Gross, B.L. 2014. Plant shoot tip response to treatment with plant vitrification solution 2. Acta Horticulturae. 1039:81-84.

Interpretive Summary: Security back-ups are needed to ensure that collections of plant genetic resources are safe from environmental disasters. Plant cryopreservation has become an effective method to conserve desirable cultivars of vegetatively propagated plant species. In many cases, excised shoot tips are treated with cryoprotectant solutions to prevent the formation of lethal ice during the freezing process. Plant vitrification solution 2 (PVS2) is a highly effective cryoprotectant solution that desiccates, permeates, and changes the freezing properties within shoot tips. We summarize a series of experiments that have been performed to better understand the mechanism by which PVS2 interacts with plant cells. Our program has measured toxicity, calorimetric, structural, and genetic responses to PVS2 and LN treatments within the shoot tips of model systems. Despite the toxicity of PVS2, it remains a highly effective vitrification solution for plant shoot tips systems.

Technical Abstract: Plant cryopreservation has become an effective method to conserve desirable genotypes of vegetatively propagated plant species. In many cases, excised shoot tips are treated with cryoprotectant solutions to prevent the formation of lethal ice during the freezing process. Plant vitrification solution 2 (PVS2) is a highly effective cryoprotectant solution that desiccates, permeates, and changes the freezing properties within shoot tips. We summarize a series of experiments that have been performed to better understand the mechanism by which PVS2 interacts with plant cells. Our program has measured toxicity, calorimetric, structural, and genetic responses to PVS2 and LN treatments within the shoot tips of model systems. Despite the toxicity of PVS2, it remains a highly effective vitrification solution for plant shoot tips systems.

Last Modified: 10/21/2014
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