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United States Department of Agriculture

Agricultural Research Service

Research Project: BITING ARTHROPODS: INTEGRATED PEST MANAGEMENT

Location: Mosquito and Fly Research Unit

Title: Blood Meal Analysis of Mosquitoes Involved in a Rift Valley fever Outbreak

Authors
item Lutomiah, J -
item Omondi, D -
item Masiga, D -
item Mutai, C -
item Mireji, P -
item Ongus, J -
item Linthicum, Kenneth
item Sang, Rosemary -

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: October 31, 2013
Publication Date: N/A

Technical Abstract: Background: Rift Valley fever (RVF) is a zoonosis of domestic ruminants in Africa. Bloodfed mosquitoes collected during the 2006-2007 RVF outbreak in Kenya were analyzed to determine the virus infection status and animal source of the bloodmeals. Bloodmeals from individual mosquito abdomens were screened for viruses using Vero cells and RT-PCR. DNA was also extracted and the cytochrome c oxidase 1 (CO1) and cytochrome b (cytb) genes amplified by PCR. The predominant species in Garissa were Ae. ochraceus, (n=561, 76%) and Ae. mcintoshi, (n=176, 24%), and Ma. uniformis, (n=24, 72.7%) in Baringo. Ae. ochraceus fed on goats (37.6%), cattle (16.4%), donkeys (10.7%), sheep (5.9%) and humans (5.3%). Ae. mcintoshi fed on the same animals in almost equal proportions. In Baringo, Ma. uniformis fed on sheep (38%), frogs (13%), duikers (8%), cattle (4%), goats (4%) and unidentified hosts (29%); with infection and dissemination rates of 25% (6/24) and 83.3% (5/6) respectively. This study has demonstrated that during the RVFVoutbreak sheep and goats were the main amplifiers of these viruses respectively.

Last Modified: 9/20/2014
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